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recombinant human cd73  (Sino Biological)


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    Structured Review

    Sino Biological recombinant human cd73
    Recombinant Human Cd73, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cd73/Human+CD73+%2F+NT5E+Protein/pm41986718-757-2-5
    Average 94 stars, based on 6 article reviews
    recombinant human cd73 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: A Novel Anti-CD73 Antibody That Selectively Inhibits Membrane CD73 Shows Antitumor Activity and Induces Tumor Immune Escape
    Article Snippet: .. Beads were then washed in PBS and incubated with 2 mg cell lysate or 0.75 μg recombinant soluble His-tagged human CD73 (Sino Biologicals) at 4 °C overnight. ..

    Recombinant:

    Article Title: A Novel Anti-CD73 Antibody That Selectively Inhibits Membrane CD73 Shows Antitumor Activity and Induces Tumor Immune Escape
    Article Snippet: .. Beads were then washed in PBS and incubated with 2 mg cell lysate or 0.75 μg recombinant soluble His-tagged human CD73 (Sino Biologicals) at 4 °C overnight. ..

    Derivative Assay:

    Article Title: Oxford nanopore sequencing enables rapid discovery of single-domain antibodies from phage display libraries.
    Article Snippet: .. We first conducted a preliminary assessment of the data quality derived from nanopore sequencing of a phage-displayed VHH library prepared from the lymphocytes of llamas (Lama glama) immunized with human CD73 (Cat. No. 10904-H08H; Sino Biological, Beijing, China) and human HER2 (Cat. No. HE2-H5225; ACROBiosystems, Beijing, China) as described previously [7,8]. .. All animal procedures were conducted using protocols approved by the National Research Council Canada Animal Care Committee and in accordance with the guidelines set out in the OMAFRA Animals for Research Act, R.S.O.

    Nanopore Sequencing:

    Article Title: Oxford nanopore sequencing enables rapid discovery of single-domain antibodies from phage display libraries.
    Article Snippet: .. We first conducted a preliminary assessment of the data quality derived from nanopore sequencing of a phage-displayed VHH library prepared from the lymphocytes of llamas (Lama glama) immunized with human CD73 (Cat. No. 10904-H08H; Sino Biological, Beijing, China) and human HER2 (Cat. No. HE2-H5225; ACROBiosystems, Beijing, China) as described previously [7,8]. .. All animal procedures were conducted using protocols approved by the National Research Council Canada Animal Care Committee and in accordance with the guidelines set out in the OMAFRA Animals for Research Act, R.S.O.

    Transfection:

    Article Title: A Novel Anti-CD73 Antibody That Selectively Inhibits Membrane CD73 Shows Antitumor Activity and Induces Tumor Immune Escape
    Article Snippet: Cell line identification was performed by STR-PCR (Eurofins, Ebersberg, Germany). .. The 293T cells were transfected with an expression plasmid, coding for human CD73 (human CD73 ORF cDNA clone expression plasmid, HIS-tagged, (Sino Biologicals, Beijing, China) using LipofectamineTM 2000 transfection reagent (ThermoFisher Scientific, Menzel, Germany) according to manufacturer’s instructions. ..

    Expressing:

    Article Title: A Novel Anti-CD73 Antibody That Selectively Inhibits Membrane CD73 Shows Antitumor Activity and Induces Tumor Immune Escape
    Article Snippet: Cell line identification was performed by STR-PCR (Eurofins, Ebersberg, Germany). .. The 293T cells were transfected with an expression plasmid, coding for human CD73 (human CD73 ORF cDNA clone expression plasmid, HIS-tagged, (Sino Biologicals, Beijing, China) using LipofectamineTM 2000 transfection reagent (ThermoFisher Scientific, Menzel, Germany) according to manufacturer’s instructions. ..

    Plasmid Preparation:

    Article Title: A Novel Anti-CD73 Antibody That Selectively Inhibits Membrane CD73 Shows Antitumor Activity and Induces Tumor Immune Escape
    Article Snippet: Cell line identification was performed by STR-PCR (Eurofins, Ebersberg, Germany). .. The 293T cells were transfected with an expression plasmid, coding for human CD73 (human CD73 ORF cDNA clone expression plasmid, HIS-tagged, (Sino Biologicals, Beijing, China) using LipofectamineTM 2000 transfection reagent (ThermoFisher Scientific, Menzel, Germany) according to manufacturer’s instructions. ..



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    (a) Immunofluorescence staining showing expression of Vimentin (green) and CD105 (red) in DPSC and SHED compared with HaCaT epithelial controls. (b) STRO-1 (green) immunostaining in SHED. Nuclei were counterstained with DAPI (blue). Scale bar = 100μm (c) Representative flow cytometry analyses demonstrating expression of mesenchymal stem cell markers <t>(CD73,</t> CD90, CD105, HLA-ABC) and absence of hematopoietic markers (CD34, CD45). All experiments were perfomed at passage 5 (d) Growth curves comparing cumulative population doublings of DPSC and SHED derived from four independent donors across serial passages. SHED displayed enhanced proliferative capacity and reduced donor-to-donor variability.
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    Image Search Results


    (a) Immunofluorescence staining showing expression of Vimentin (green) and CD105 (red) in DPSC and SHED compared with HaCaT epithelial controls. (b) STRO-1 (green) immunostaining in SHED. Nuclei were counterstained with DAPI (blue). Scale bar = 100μm (c) Representative flow cytometry analyses demonstrating expression of mesenchymal stem cell markers (CD73, CD90, CD105, HLA-ABC) and absence of hematopoietic markers (CD34, CD45). All experiments were perfomed at passage 5 (d) Growth curves comparing cumulative population doublings of DPSC and SHED derived from four independent donors across serial passages. SHED displayed enhanced proliferative capacity and reduced donor-to-donor variability.

    Journal: bioRxiv

    Article Title: Human SHED-derived extracellular cues activate a specialized neuroprotective and regenerative program in developing retinal ganglion cells

    doi: 10.64898/2026.06.25.733625

    Figure Lengend Snippet: (a) Immunofluorescence staining showing expression of Vimentin (green) and CD105 (red) in DPSC and SHED compared with HaCaT epithelial controls. (b) STRO-1 (green) immunostaining in SHED. Nuclei were counterstained with DAPI (blue). Scale bar = 100μm (c) Representative flow cytometry analyses demonstrating expression of mesenchymal stem cell markers (CD73, CD90, CD105, HLA-ABC) and absence of hematopoietic markers (CD34, CD45). All experiments were perfomed at passage 5 (d) Growth curves comparing cumulative population doublings of DPSC and SHED derived from four independent donors across serial passages. SHED displayed enhanced proliferative capacity and reduced donor-to-donor variability.

    Article Snippet: Antibodies against the following human antigens were used: CD105-FITC (Miltenyi Biotect, Bergisch Gladbach, Germany, cat# 130-112-327, 1:50), CD90-FITC (Miltenyi Biotec, cat# 130-114-901, 1:50), CD44-VioBlue (Miltenyi Biotec, cat# 130-113-906, 1:50), CD73-APC (Miltenyi Biotec, cat# 130-111-909, 1:50), MSC Phenotyping Cocktail-PE (CD34, CD14, CD19, CD45, Miltenyi Biotec cat# 130-125-285, dilution according to the manufacturer’s instructions).

    Techniques: Immunofluorescence, Staining, Expressing, Immunostaining, Flow Cytometry, Derivative Assay